Journal: Vaccines

Article Title: Molecular Events in Immune Responses to Sublingual Influenza Vaccine with Hemagglutinin Antigen and Poly(I:C) Adjuvant in Nonhuman Primates, Cynomolgus Macaques

doi: 10.3390/vaccines12060643

Figure Lengend Snippet: Points in time for sublingual vaccinations and assessments of anti-HA (hemagglutinin) antibodies, blood tests, plasma cytokines, quantitative reverse transcription PCR (RT-qPCR), and DNA microarray analyses. RT-qPCR and DNA microarray analyses were conducted with RNA isolated from white blood cells (WBC). Vaccinations were performed four times at 0 (1st), 6 (2nd), 18 (3rd), and 30 (4th) weeks. Arrows indicate sampling timepoints for each assay.

Article Snippet: The labeled cRNA was subsequently fragmented using the Gene Expression Hybridization Kit (Agilent Technologies, Santa Clara, CA, USA) and then set onto Whole Human Genome DNA Microarray 4× 44K v2 slides (Agilent Technologies, Santa Clara, CA, USA).

Techniques: Reverse Transcription, Quantitative RT-PCR, Microarray, Isolation, Sampling

Journal: BMC Immunology

Article Title: Differential effects of Paclitaxel on dendritic cell function

doi: 10.1186/1471-2172-11-14

Figure Lengend Snippet: Microarray analysis of DC exposed to LPS 1 μg/ml or paclitaxel 100 μM for 2 hours.

Article Snippet: This was used as a probe on the Whole Human Genome Microarray (4 × 44 K) slide (Agilent).

Techniques: Microarray

Journal: Cellular signalling

Article Title: ERK5 induces ankrd1 for catecholamine biosynthesis and homeostasis in adrenal medullary cells.

doi: 10.1016/j.cellsig.2015.12.016

Figure Lengend Snippet: Identification of ERK5-dependent genes. (A) PC12 cells were stimulated with or without NGF (100 ng/ml) for 4 h in the presence or absence of U0126 (30 μM) and BIX02189 (30 μM), then gene expression was examined by microarray analysis. Genes that were up-regulated more than three fold by NGF were defined as NGF-stimulated genes. Among them, genes whose expression was inhibited by more than 50% by U0126 and BIX02189 were defined as ERK1/2-dependent genes and ERK5-dependent genes, respectively. (B) PC12 cells were incubated with or without NGF (100 ng/ml) for 5 min in the presence or absence of U0126 (U, 30 μM) and BIX02189 (BIX, 30 μM), then phosphorylations of ERK5 and ERK1/2 were examined. These samples were prepared at the same time when samples for microarray analysis were prepared. (C) Examples of ERK5-specific genes (dark gray), ERK5 and ERK1/2-dependent genes (light gray) and NGF-independent genes (white).

Article Snippet: Microarray slides (whole rat genome oligo microarray 4 × 44 k, Agilent Technology) were used for the transcript analysis.

Techniques: Expressing, Microarray, Incubation

Journal: Data in Brief

Article Title: Expression profiling of long noncoding RNAs in neonatal and adult mouse testis

doi: 10.1016/j.dib.2015.06.004

Figure Lengend Snippet: Validation of microarray results by qRT-PCR [3] .

Article Snippet: 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide (Mouse Stringent LncRNA microarray, 4×44 K, ArrayStar).

Techniques: Biomarker Discovery, Microarray

Journal: Data in Brief

Article Title: Expression profiling of long noncoding RNAs in neonatal and adult mouse testis

doi: 10.1016/j.dib.2015.06.004

Figure Lengend Snippet: List of primers used in the validation of microarray results by qRT-PCR.

Article Snippet: 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide (Mouse Stringent LncRNA microarray, 4×44 K, ArrayStar).

Techniques: Biomarker Discovery, Microarray, Sequencing, Amplification

Journal: Data in Brief

Article Title: Expression profiling of long noncoding RNAs in neonatal and adult mouse testis

doi: 10.1016/j.dib.2015.06.004

Figure Lengend Snippet:

Article Snippet: 100 μl of hybridization solution was dispensed into the gasket slide and assembled to the lncRNA expression microarray slide (Mouse Stringent LncRNA microarray, 4×44 K, ArrayStar).

Techniques: Microarray, Comparison

Journal: Frontiers in Pharmacology

Article Title: Calculus Bovis Sativus Improves Bile Acid Homeostasis via Farnesoid X Receptor-Mediated Signaling in Rats With Estrogen-Induced Cholestasis

doi: 10.3389/fphar.2019.00048

Figure Lengend Snippet: DNA microarray analysis of non-cholestatic, 17α-ethinylestradiol (EE) and EE+CBS rat livers. (A) Venn diagram analysis of differentially expressed genes of non-cholestatic, EE and EE+CBS groups. (B) Heat map of differentially expressed genes in non-cholestatic, EE and EE+CBS groups (C) gene ontology (GO) analysis of differentially expressed genes (D) Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis of differentially expressed genes. Arrows point to the pathways related to bile acid (BA) homeostasis; ( n = 3). Abbreviations: BP, biological process; CC, cellular component; MF, molecular function. Interpretation: GO:0042493, response to drug; GO:1903298, negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway; GO:0000122, negative regulation of transcription from RNA polymerase II promoter; GO:0050821, protein stabilization; GO:0060662, salivary gland cavitation; GO:0070062, extracellular exosome; GO:0031012, extracellular matrix; GO:0005790, smooth endoplasmic reticulum; GO:0005925, focal adhesion; GO:0005794, Golgi apparatus; GO:0005515, protein binding; GO:0019900, kinase binding; GO:0042803, protein homodimerization activity; GO:0005506, iron ion binding; and GO:0004305, ethanolamine kinase activity.

Article Snippet: Each slide was hybridized with Agilent Whole Rat Genome Oligo Microarray (4 × 44K).

Techniques: Microarray, Protein Binding, Binding Assay, Activity Assay